Phenotypic characterization of HLA-C*07:02/IE-1^MAGE- and HLA-B*07:02/pp65 multimer⁺ T cells.

<p>Phenotypic analysis of HLA-C*07:02/IE-1^MAGE-- or HLA-B*07:02/pp65-specific CD8⁺ naïve (CCR7⁺CD45RO^-), central memory (CCR7⁺CD45RO⁺), effector memory (CCR7^-CD45RO⁺) and effector (CCR7^-CD45RO^-) T cells. <b>(A)</b> Exemplary multimer CD8⁺ T cell stainings and <b>(B)</b> differentiation phenotypes for the corresponding epitopes are shown. <b>(C)</b> Central memory, <b>(D)</b> effector memory and <b>(E)</b> effector CD8⁺ T cell subset frequencies (%) from HLA-C*07:02/ HLA-B*07:02 donors are compared for the respective epitopes. T cells were uniformly pre-gated on living CD3⁺/CD8⁺ lymphocytes. Statistical analyses were performed with the Mann-Whitney <i>U</i> test. * = p < 0.05, ** = p < 0.01, *** = p < 0.001.