Chemical inhibition of JNK reduces egg tanning and hatching.

<p>(A) Total oviposited eggs from control, DMSO, or JNK inhibitor injected female mosquitoes (Scale bar = 0.5 mm.) (B) Melanized and non-melanized eggs (number in the parentheses denotes the total number of eggs counted). Y-axis is the percentage of melanized eggs of total eggs counted. (C) Eggs from control, DMSO, or JNK inhibitor (JNKi)-treated mosquitoes subjected to deoxygenation-induced hatching for melanized (MZ) and non-melanized (non-MZ) eggs. Number in the parentheses denotes total number of eggs examined. (D) Eggs from control, DMSO-, and JNK inhibitor-treated mosquitoes separated into melanized (MZ) and non-melanized (non-MZ) eggs. Scale bar = 10 μm. (E) Percentage of eggs with complete micropylar pore formation (Y-axis). Number in the parentheses denotes the number of eggs with complete micropylar formation divided by the total number counted.