Metabolic characterization of dip coated cells.

<p>Bioenergetics were assessed with a Seahorse XFp for both untreated cells and cells which were processed via dip-coating technique (100 mm/min, 1.2 M trehalose solution), rehydrated immediately, and cultured for 14 days. <b>A)</b> Respiration (oxygen consumption rate) is shown, with legend titles referring to readings after specified reagent injections. <b>B)</b> Glycolysis (extracellular acidification rate) is shown similarly to A. <b>C)</b> Spare respiratory capacity is a (%) ratio between FCCP and Oligomycin groups in A. <b>D)</b> Glycolytic reserve is the (%) ratio between oligomycin and glucose groups in B. There was no statistical difference between treated and untreated groups. Error bars show +SD, n = 6.