Detection of anti-r<i>Pf</i>PMT antibodies in a human anti-malaria hyperimmune antibody pool.

<p>A human anti-malaria hyperimmune antibody pool was passed over a r<i>Pf</i>PMT affinity column. The affinity purified anti-r<i>Pf</i>PMT human antibodies (100 ng) were used to detect each of the recombinant PMT orthologues or each of the PMT peptide epitopes coated directly onto ELISA plates at 100 ng per well. The pool before (antibody pool) and the pool after (depleted antibody pool) passing the human antibodies over the r<i>Pf</i>PMT affinity resin were used at 10 μg. All readings were done in triplicate with standard deviations shown. A cut-off value for positive reactions of three times the standard deviation of the background control was included as a horizontal dashed line. Student’s t-test with <i>p</i> ≤ 0.05 and ≤ 0.001 are indicated with “*” or “**” respectively.