Additional file 2: of The hypoxic tumor microenvironment in vivo selects the cancer stem cell fate of breast cancer cells

Figure S2. Examination of HIF-1α and HIF-2α in sorted tumor cells from the MDA-MB-231/HRE-EGFP xenografts, and inhibition of AKT phosphorylation by LY294002. (A) Dynamic regulation of HIF-1α and HIF-2α proteins in response to hypoxia and re-oxygenation is examined in Western blots. These results show that the O2-dependent regulation of HIF-α stability in the sorted EGFP+ and EGFP− tumor cells is normal and comparable to the parental MDA-MB-231 cells. (B) The PI3K-specific inhibitor LY294002 (20 μM) blocks AKT phosphorylation in the sorted EGFP+ and EGFP− MDA-MB-231 cells. (TIFF 2045 kb