Investigation of autophagy.

<p>(A) Representative western blot of LC3B-I, LC3B-II and GAPDH loading control in DANs derived from 4 iPD patients and 4 healthy controls. Labels C/C (risk allele) and T/T (non-risk allele) denote the genotype of iPD patients at LRRK2-RS1491923. mDANs were treated with a DMSO vehicle control or 100 nM bafilomycin (baf) for 24 h. (B) Western blots from three independent experiments (n = 3) were quantified by densitometry and LC3B-II normalized to a loading control. All measurements are shown. Data is grouped as healthy vs iPD as well as iPD stratified for T/T and C/C genotype. (C) Ratio of LC3B-II (normalized to loading control) following bafilomycin treatment/untreated. (D) Representative western blot of LAMP1 and α-tubulin loading control in mDANs derived from iPD patients and healthy controls. (E) Western blots from three independent experiments (n = 3) were quantified by densitometry and LAMP1 normalized to a loading control. All data is shown, including data stratified for T/T and C/C genotype. (F) Ratio of LAMP1 (normalized to loading control) following bafilomycin treatment/untreated.