Quantification of mitochondrial protein levels.

<p>(A) Representative western blot of ATP5A and Vinculin loading control in mDANs derived from 4 iPD patients and 4 healthy controls. Labels C/C (risk allele) and T/T (non-risk allele) denotes the genotype of iPD patients at LRRK2-RS1491923. DANs were treated with a DMSO vehicle control or 100 nM rotenone (rot) for 24 h. (B) Western blots from three independent experiments (n = 3) were quantified by densitometry and ATP5A normalized to a loading control. All data points are reported. Data is grouped as healthy vs iPD as well as iPD data stratified for T/T and C/C genotype. (C) Ratio of ATP5A (normalized to loading control) following rotenone treatment/untreated. (D) Representative western blot of TOMM20 and β-actin loading control in DANs derived from iPD patients and healthy controls. mDANs were treated with a DMSO vehicle control or 1μM valinomycin (val) for 24 h. (E) Western blots from three independent experiments (n = 3) were quantified by densitometry and TOMM20 normalized to a loading control. Data stratified for T/T and C/C genotype for both the untreated and rotenone treated condition are also shown. (F) Ratio of TOMM20 (normalized to loading control) following valinomycin treatment/untreated.