<i>med8</i> and <i>med25</i> mutants affect plant resistance to <i>B</i>. <i>cinerea</i> by independent and additive means.

<p>(A) (B) Disease symptoms and lesion sizes on the leaves of the <i>B</i>. <i>cinerea</i>-infected WT, <i>med8</i>, <i>med25</i>, and <i>med8med25</i> at 3 d. The disease assay was performed by drop inoculation of <i>B</i>. <i>cinerea</i> on the leaves of soil-grown plants. The infected leaves were photographed and bar = 4 mm (A). Average values and SEM from relative values obtained in four biological replicates were plotted on the graph (B). A minimum of 10 leaves for each genotype was used for each biological replicate, and the disease assay was repeated at least four times, with similar results. The mean values followed by different letters represent significant differences (<i>P</i>< 0.01, Student’s <i>t</i>-test). (C) (D) RT-qPCR analysis of <i>ERF1</i> and <i>PDF1</i>.<i>2</i> RNA levels in the WT, <i>med8</i>, <i>med25</i>, and <i>med8med25</i> leaves of soil-grown plants at 36 hpi after inoculation with <i>B</i>. <i>cinerea</i>. Expression of <i>ERF1</i> and <i>PDF1</i>.<i>2</i> was normalized against the constitutively expressed <i>Actin7</i>. Average values and SEM from relative values obtained in four biological replicates were plotted on the graph. The mean values followed by different letters represent significant differences (<i>P</i>< 0.01, Student’s <i>t</i>-test).