Apoptosis of cancer cells treated with TRAIL and CQ.

<p>(A) Two cancer cell lines were cultured with the indicated doses of TRAIL and CQ for 48 h. After staining with Annexin V-FITC/PI, the cells were subjected to flow cytometry. The data are the means and standard deviation (SD) of those from three wells. *<i>P</i><0.05. **<i>P</i><0.01. (B) Representative results. The numbers indicate the proportions of cells in each subset. (C) Two cancer cell lines were cultured with CQ (200 nM for MiaPaCa-2 cells, 700 nM for Panc-1 cells), TRAIL (10 ng/mL for MiaPaCa-2 cells, 100 ng/mL for Panc-1 cells), or both for 24 h. The expression levels of caspase-3, caspase-8, and caspase-9 were measured. α-Tubulin served as a control.