Effect of p53 siRNA on ROS production and cell apoptosis.

<p>(A) Parental HT-29 cells (P) were treated with scramble (Scr) or p53 siRNA for 72 hr and p53 protein was determined by Western blot. Untreated NAT1 knockout cells (KO) were also analyzed for comparison. (B) Effect of p53 siRNA on ROS production in Parental cells grown in low (1 mM) glucose. NAT1 knockout cells (closed bars) are shown for comparison. Data were expressed relative to Parental cells treated with scramble siRNA. (C) Effect of p53 siRNA on apoptosis in Parental cells grown in low (1 mM) glucose. Data are mean ± sem, n = 3. Asterisk indicates significant difference by one-way ANOVA.