Differential response of MyD88- or TRIF-deficient DCs to apoptotic cell phagocytosis.

<p>(A) WT, <i>Myd88</i>^-/- and <i>Trif</i>^-/- BMDCs were stimulated with apoptotic splenocytes for 6 or 24 hours and the expression of <i>Tnf</i>, <i>Il10</i> and <i>Ido</i> mRNAs was measured by qRT-PCR. Data shown are representative of three independent experiments. (B) The expression of NF-κB proteins was assessed by immunoblotting with specific antibodies in cytoplasmic and nuclear extracts from BMDCs stimulated with apoptotic splenocytes for 4 hours. Tubulin and HDAC1 were used as loading controls. Blots are representative of three independent experiments. (C) The mRNA expression of the indicated genes was measured by qRT-PCR in peritoneal cells collected from MyD88^CD11c-KO, TRIF^CD11c-KO and Cre negative littermate control mice (n = 4 per genotype) 18 hours after i.p. injection of 10⁷ apoptotic thymocytes or medium-only. Data are representative of two independent experiments. (D) The mRNA and protein expression of IDO was analyzed by qRT-PCR and immunoblotting respectively in WT, <i>Myd88</i>^-/- and <i>Trif</i>^-/- BMDCs that were stimulated with primary apoptotic islets (immunoblot was performed on protein extracts prepared from islets stimulated for 12 hours with STZ). Data are representative of two independent experiments.