<i>Prss56</i>^-/- eyes exhibit reduced ocular axial length and hyperopia.

<p>(<b>A</b>) Representative images of slit-lamp examination by broad-beam illumination to assess ocular structures including the iris, pupil, and lens at 1 and 3 months of age. <i>Prss56</i>^<i>-/-</i> eyes did not exhibit any obvious structural abnormalities and were indistinguishable from <i>Prss56</i>^<i>+/-</i> eyes. (<b>B</b>) Representative OCT images demonstrating a reduction in ocular size in <i>Prss56</i>^<i>-/-</i> compared to <i>Prss56</i>^<i>+/-</i> mice (shown are P30 eyes). The red, blue and yellow lines indicate ocular axial length (AL), vitreous chamber depth (VCD) and anterior chamber depth (ACD), respectively. (<b>C</b>-<b>D</b>) <i>Prss56</i>^<i>-/-</i> eyes exhibit a modest but highly significant reduction in axial length (<b>C</b>) and equatorial diameter (<b>D</b>) at P15 and P25. (<b>E</b>) A significant reduction in VCD and increase in ACD was detected in <i>Prss56</i>^<i>-/-</i> compared to <i>Prss56</i>^+/- eyes (shown are data from P30 eyes). (<b>F</b>) Consistent with reduced ocular size, <i>Prss56</i>^<i>-/-</i> mice display a hyperopic refraction compared to <i>Prss56</i>^<i>+/-</i> littermates (shown are data from 2-months old mice). (<b>G</b>) Retinal thickness was significantly increased in <i>Prss56</i>^<i>-/-</i> compared to <i>Prss56</i>^<i>+/-</i> eyes. Values are presented as mean ± SD, *** p<0.001, t-test. <b>C</b> and <b>D:</b> N > 10 per group; <b>E</b> and <b>F:</b> N ≥ 6 per group; and <b>G:</b> N≥ 4. (<b>H</b>-<b>I</b>) Representative B-scan images of eyes from an unaffected individual (<b>H</b>) and an individual with a <i>PRSS56</i> mutation (<b>I</b>). VCD is substantially reduced in the eye of the individual carrying a mutant <i>PRSS56</i> allele compared to a normal emmetropic eye.