Motor neuron development is normal in <i>casy-1 mutants</i>.

<p>(A) Representative fluorescent images of WT and <i>casy-1</i> mutant animals expressing GFP in all GABAergic neurons (<i>juIs76</i> [P<i>unc-25</i>:: GFP]). The number of cell bodies and axonal commissures were largely normal in <i>casy-1</i> mutant animals (n-25) analyzed. Scale bar, 8μm. (B) CASY-1 functions in the mature nervous system to regulate synaptic transmission at the NMJ. Expression of CASY-1A isoform just three hours before the assay using a heat- shock promoter (<i>hsp16</i>.<i>2</i>) completely rescues the Aldicarb hypersensitivity phenotype seen in <i>casy-1</i> mutant animals. The number of assays (~20 <i>C</i>. <i>elegans</i>/assay) is indicated for each genotype. (C) The C-terminal of the CASY-1A isoform is required to regulate synaptic transmission at the NMJ. Transgenic lines expressing either the CASY-1A N-terminal (ΔC) or C-terminal (ΔN) alone expressed under <i>ins-1</i> promoter suggest that the CASY-1A C- terminal is sufficient to rescue the Aldicarb hypersensitivity in <i>casy-1</i> mutants. Assays were done 3 times as indicated in Figures (~20 <i>C</i>. <i>elegans</i>/ assay). Data are represented as mean ± S.E.M. (***<i>p</i><0.0001 using one-way ANOVA and Bonferroni's Multiple Comparison Test). “ns” indicates not significant in all Figures.