The investigation of chemical variation in the stems and rhizomes of cissampelos capensis l. f. (menispermaceae) the Eastern Cape medicinal plant

Cissampelos capensis L. f. (Menispermaceae) commonly known as “Mayisake” (Xhosa) and “Dawidjieswortel” (Afrikaans) is the most commonly used medicinal plant of the Menispermaceae family in South Africa. The rhizomes of Cissampelos capensis are the most used part of the plant to treat illnesses. This poses a threat to the ongoing conservation of this valuable endemic species. Thus, we studied the chemical variation and subsequently the bioactivity in the stems and rhizomes of Cissampelos capensis, the Eastern Cape medicinal plant, to suggest the possible use of the stems for medicinal purposes. We studied the variation in the chemical composition in the stems and the rhizomes by employing four different extraction methods; the methanolic-, non-polar-, alkaloidal- and Total Tertiary Alkaloidal (TTA) to extract compounds from Cissampelos capensis. Employing various chromatographic techniques, HPLC, NMR and LC/MS to investigate the chemical variation and the in vitro assays for the crude materials of the stems and rhizomes of Cissampelos capensis. We successfully documented a library of the phytochemicals content in Cissampelos capensis from three different regions in the Eastern Cape. We could profile different extraction methods, with the TTA extraction delivering the best comparative profile. Employing LC/MS and HPLC we identified eleven known alkaloidal compounds in the stems and rhizomes. We also isolated a known alkaloid, pronuciferine, from the TTA extract and a crystal structure of proto-quericitol from the methanolic extract, which was isolated for the first time from Cissampelos capensis. Preliminary in vitro results suggested anti-inflammatory activity in the methanolic extracts of the stems and rhizomes, while the TTA extracts showed some cytotoxicity. Finally, through analytical HPLC analysis this study found as high as 31% similarity between the chemical variation of the stems and rhizomes, but key alkaloids were not always present, indicating more work is needed before the stems can be recommended as a replacement for the rhizomes