Semeiology and pattern analysis in melanocytic lesions

Melanocytic lesion exploration is conducted by the examination of mosaics and/or series of consecutive high-resolution images from the surface to the dermis up to the loss of resolution (approximately up to 200 μm). Mosaics are usually acquired at three levels (superficial layers, dermal-epidermal junction, upper dermis) for the evaluation of general epidermal pattern, frequencies and distribution of pagetoid cells, regularity of the DEJ architecture, presence and distribution of nests, and structures within the papillary dermis. High-resolution images are employed for the evaluation of cyto-architectural aspects