Role of ACTL6A in human prostate cancer cell

[[abstract]]Prostate cancer (PCa) incidence is the 2nd most common male cancer overall in the world. PCa is the 6th most common cancer in Taiwan. Actin-like protein 6A (ACTL6A) gene encodes a family member of actin-related proteins (ARPs), which are involved in vesicular transport, spindle orientation, nuclear migration and chromatin remodeling in cells. We compare the correlation between ACTL6A and serum prostate specific antigen (PSA). We observed that high IHC staining level of ACTL6A correlated to high serum PSA and high prostate cancer recurrence rate in PCa patients. PC-3 is PCa cell lines generated from bone metastatic PCa cells. Knockdown of ACTL6A by shRNA in PC-3 cells suppressed proliferation, migration, and invasion. Micro-Western Array analysis indicated that knock down of ACTL6A affected proteins involved in epithelial-mesenchymal transition (EMT). Orthotopic nude mice PCa model with IVIS imaging revealed that knockdown of ACTL6A reduced PCa metastasis. Our result suggested that ACTL6A is a novel oncogene for PCa and may serve as a prognostic marker for advanced PCa