Add to ORKGBy screening the phage-displayed human single chain antibody library, we have got the specific single chain antibody bound to GSH-S-DNP butyl ester as the hapten. The tertiary structure of the protein was analyzed with the aid of computer, and the results showed the CDR3 region located on the surface of the antibody. The soluble antibody was expressed in E. coli. and the active site serine was converted into selenocysteine with the chemical modifying method, which resulted in the catalytic antibody with GPx activity of 80 U/mu mol. Furthermore, the same Ping-Pong mechanism as the natural GPx was observed when the kinetic behavior of the antibody was studied
Accumulating evidence shows that glutathione peroxidase (GPX, EC.1.11.1.9), one of the most importan...
Chapter 18. Glutathione peroxidase 4 (GPx4) is a selenocysteine (Sec)-containing glutathione peroxid...
AbstractGlutathione peroxidase (GPX) protects cells against oxidative damage by catalyzing the reduc...
In order to generate catalytic antibodies with glutathione peroxidase (GPx) activity, we prepared GS...
A selenium-containing catalytic antibody (Se-4A4), prepared by converting reactive serine residues o...
Three selenium-containing catalytic antibodies mHB4, mHB5 and mHB7 which acted as mimics of cytosoli...
Kinetics and molecular mechanisms of GPx-type enzymes are reviewed with emphasis on structural featu...
A panel of catalytic antibodies which catalyze ester hydrolysis, transesterification, porphyrin meta...
The vast binding repertoire of the immune system has been exploited for the generation of tailor-mad...
The discoveries leading to our present understanding of the glutathione peroxidases (GPxs) are recal...
Plasma glutathione peroxidase (GPx3) belongs to the GPx superfamily, and it is the only known secret...
The vast binding repertoire of the immune system has been exploited for the generation of tailor-mad...
Single-chain fragment variable(ScFv) antibodies with the substrate binding sites were obtained by re...
A review with 10 refs. Catalytic antibodies have created a new dimension in protein chem. In these s...
A review with 10 refs. Catalytic antibodies have created a new dimension in protein chem. In these s...
Accumulating evidence shows that glutathione peroxidase (GPX, EC.1.11.1.9), one of the most importan...
Chapter 18. Glutathione peroxidase 4 (GPx4) is a selenocysteine (Sec)-containing glutathione peroxid...
AbstractGlutathione peroxidase (GPX) protects cells against oxidative damage by catalyzing the reduc...
In order to generate catalytic antibodies with glutathione peroxidase (GPx) activity, we prepared GS...
A selenium-containing catalytic antibody (Se-4A4), prepared by converting reactive serine residues o...
Three selenium-containing catalytic antibodies mHB4, mHB5 and mHB7 which acted as mimics of cytosoli...
Kinetics and molecular mechanisms of GPx-type enzymes are reviewed with emphasis on structural featu...
A panel of catalytic antibodies which catalyze ester hydrolysis, transesterification, porphyrin meta...
The vast binding repertoire of the immune system has been exploited for the generation of tailor-mad...
The discoveries leading to our present understanding of the glutathione peroxidases (GPxs) are recal...
Plasma glutathione peroxidase (GPx3) belongs to the GPx superfamily, and it is the only known secret...
The vast binding repertoire of the immune system has been exploited for the generation of tailor-mad...
Single-chain fragment variable(ScFv) antibodies with the substrate binding sites were obtained by re...
A review with 10 refs. Catalytic antibodies have created a new dimension in protein chem. In these s...
A review with 10 refs. Catalytic antibodies have created a new dimension in protein chem. In these s...
Accumulating evidence shows that glutathione peroxidase (GPX, EC.1.11.1.9), one of the most importan...
Chapter 18. Glutathione peroxidase 4 (GPx4) is a selenocysteine (Sec)-containing glutathione peroxid...
AbstractGlutathione peroxidase (GPX) protects cells against oxidative damage by catalyzing the reduc...