Disruption of P-selectin signaling modulates cell trafficking and results in improved outcomes after mouse warm intestinal ischemia and reperfusion injury
- Farmer, Douglas G.
- Anselmo, Dean
- Shen, Xiu Da
- Ke, Bibo
- Carmody, Ian C.
- Gao, Feng
- Lassman, Charles
- McDiarmid, Sue V.
- Shaw, Grey
- Busuttil, Ronald W.
- Kupiec-Weglinski, Jerzy W.
Publication date
September 2005
DOI Publisher
Ovid Technologies (Wolters Kluwer Health) ISSN
0041-1337 Citation count (estimate)
28Abstract
Background. This study analyzes the role of T lymphocytes and neutrophils (PMN) in intestinal ischemia and reperfusion injury (IRI) using either P-selectin blockade or elimination. Methods. Using a model of severe mouse warm intestinal IRI, the following groups were performed: group 1: wild type C57BL6 no treatment; group 2: wild type treated with r-PSGL1-Ig; group 3: C57BL6 genetically deficient in P-selectin. Survival was assessed at day 7; intestine was assayed for histopathology, apoptosis, myeloperoxidase (MPO), inflammatory cytokines, hemoxygenase-1 (HO-1), and CD3 lymphocytes. Standard statistical comparison was undertaken. Results. The survival was significantly (
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