True Molecular Scale Visualization of Variable Clustering Properties of Ryanodine Receptors
- Isuru Jayasinghe
- Alexander H. Clowsley
- Ruisheng Lin
- Tobias Lutz
- Carl Harrison
- Ellen Green
- David Baddeley
- Lorenzo Di Michele
- Christian Soeller
DOIAbstract
Summary: Signaling nanodomains rely on spatial organization of proteins to allow controlled intracellular signaling. Examples include calcium release sites of cardiomyocytes where ryanodine receptors (RyRs) are clustered with their molecular partners. Localization microscopy has been crucial to visualizing these nanodomains but has been limited by brightness of markers, restricting the resolution and quantification of individual proteins clustered within. Harnessing the remarkable localization precision of DNA-PAINT (<10 nm), we visualized punctate labeling within these nanodomains, confirmed as single RyRs. RyR positions within sub-plasmalemmal nanodomains revealed how they are organized randomly into irregular clustering patterns leaving ...
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