Penyakit Layu Bakteri Stewart pada Jagung di Bali

<p>Stewart’s wilt is a serious disease of sweet corn (<em>Zea mays</em>). The typical symptoms of the disease are pale-green to yellow linear streaks parallel to the veins. The symptoms were observed on sweet corn in Denpasar, Tabanan, Gianyar, and Karangasem areas during a survey in 2015. Pathogen detection based on a polymerase chain reaction was carried out using total DNA obtained from symptomatic leaf samples and the pairs of primers, CPSL1/CPSR2c. The expected sized (~1100 bp) amplicon was detected in samples from Denpasar. Sequence analysis confirmed that Stewart’s wilt disease symptoms are caused by <em>Pantoea stewartii </em>subsp. <em>stewartii</em>. Nucleotide sequence and phylogenetic analysis showed that <em>P. stewartii </em>subsp. <em>stewartii </em>from Bali has high homology (98.97-99.08 %) and placed in the same clade with isolates from Canada, USA and Japan. This is the first report of <em>P. stewarti </em>subsp. <em>stewartii </em>on corn in Bali.