Computer simulation of genetically modified Aedes aegypti release methods.

Aedes aegypti mosquitoes are largely responsible for the spread of Dengue Fever and Zika virus. Field trials of genetically modified male Aedes aegypti which produce non-viable off spring are planned in the United States. It is critical to determine the best release method which controls the wild mosquito population and minimizes the use of modified mosquitoes. A computer simulation was developed to test various release methods under different conditions. The simulation compared favorably with known field data from Brazil. All the variable release methods tested produced a 95% reduction (p<0.001) in the wild mosquito population. Both a slower changing release method and a more rapidly adjusting method without a minimum release allowed for a late increase in wild mosquito numbers. e rapidly adjusting method with a minimum released controlled the wild mosquito population while using 17% and 19% less genetically modified mosquitoes in dry and wet conditions respectively (P < 0.001)