Objective: To apply, in Brazil, the T‐cell receptor excision circles (TRECs) quantification technique using real‐time PCR in newborn screening for severe combined immunodeficiency (SCID) and assess the feasibility of implementing it on a large scale in Brazil. Methods: 8715 newborn blood samples were collected on paper filter and, after DNA elution, TRECs were quantified by real‐time PCR. The cutoff value to determine whether a sample was abnormal was determined by ROC curve analysis, using SSPS. Results: The concentration of TRECs in 8682 samples ranged from 2 to 2181 TRECs/μL of blood, with mean and median of 324 and 259 TRECs/μL, respectively. Forty‐nine (0.56%) samples were below the cutoff (30 TRECs/μL) and were reanalyzed. Four (0.05%...
BACKGROUND: Real-time quantitative PCR (qPCR) targeting a specific marker of functional T cells, the...
BackgroundAssay of T-cell receptor excision circles (TRECs) in dried blood spots obtained at birth p...
Background: Screening newborns for severe combined immunodeficiency (SCID) has become essential, sin...
AbstractObjectiveTo apply, in Brazil, the T-cell receptor excision circles (TRECs) quantification te...
Abstract Objective To apply, in Brazil, the T-cell receptor excision circles (TRECs) quantification...
AIM: Severe combined immunodeficiency (SCID) is the most severe form of primary immunodeficiency and...
Objective: To assess the feasibility of T-cell receptor excision circles (TRECs) quantification for ...
The prognosis of children with severe combined immunodeficiency (SCID) depends on a presymptomatic d...
International audienceSevere combined immunodeficiency (SCID) is characterized by a major T cell def...
textabstractBackground: Newborn screening (NBS) by quantifying T cell receptor excision circles (TRE...
Infants born with T cell lymphopenias, especially severe combined immunodeficiency (SCID) are at ris...
Newborn screening; Severe combined immunodeficiency; T-cell receptor excision circlesCribratge de na...
Severe combined immunodeficiency (SCID) is a life-threatening condition of newborns and infants caus...
Background: Quantification of T-cell-receptor-excision circles (TRECs) and kappa-deleting-recombinat...
The development of a T cell receptor excision circle (TREC) assay utilizing dried blood spots (DBS) ...
BACKGROUND: Real-time quantitative PCR (qPCR) targeting a specific marker of functional T cells, the...
BackgroundAssay of T-cell receptor excision circles (TRECs) in dried blood spots obtained at birth p...
Background: Screening newborns for severe combined immunodeficiency (SCID) has become essential, sin...
AbstractObjectiveTo apply, in Brazil, the T-cell receptor excision circles (TRECs) quantification te...
Abstract Objective To apply, in Brazil, the T-cell receptor excision circles (TRECs) quantification...
AIM: Severe combined immunodeficiency (SCID) is the most severe form of primary immunodeficiency and...
Objective: To assess the feasibility of T-cell receptor excision circles (TRECs) quantification for ...
The prognosis of children with severe combined immunodeficiency (SCID) depends on a presymptomatic d...
International audienceSevere combined immunodeficiency (SCID) is characterized by a major T cell def...
textabstractBackground: Newborn screening (NBS) by quantifying T cell receptor excision circles (TRE...
Infants born with T cell lymphopenias, especially severe combined immunodeficiency (SCID) are at ris...
Newborn screening; Severe combined immunodeficiency; T-cell receptor excision circlesCribratge de na...
Severe combined immunodeficiency (SCID) is a life-threatening condition of newborns and infants caus...
Background: Quantification of T-cell-receptor-excision circles (TRECs) and kappa-deleting-recombinat...
The development of a T cell receptor excision circle (TREC) assay utilizing dried blood spots (DBS) ...
BACKGROUND: Real-time quantitative PCR (qPCR) targeting a specific marker of functional T cells, the...
BackgroundAssay of T-cell receptor excision circles (TRECs) in dried blood spots obtained at birth p...
Background: Screening newborns for severe combined immunodeficiency (SCID) has become essential, sin...