Detection and clinical significance of rheumatoid factor, anti -CCP antibody , immunoglobulin and cytokines in serum of patients with rheumatoid arthritis

Objective: To detect the content of rheumatoid factor, ring citrulline peptide antibody (CCP), immunoglobulin (Ig) and cytokines in serum of patients with rheumatoid arthritis (RA) and explore the clinical application value of serum indexes for RA diagnosis, treatment and prognostic.Methods: A total of 98 patients with RA were selected, of which 52 cases in the activity and 46 patients in remission, 65 healthy people as controls. Contents of serum RF and Ig (IgM, IgG, IgA) in every group were detected by methods of immune scattering turbidimetry respectively; the content of serum anti-CCP antibody and cytokines IL-1β, TNF-α, IFN-γ and IL-4 were measured by methods of enzyme-linked immunosorbent respectively. Change of detection index levels between groups were compared and the correlation between cytokines and RF, anti CCP, Ig and DAS28 score in patients with RA were analyzed. Results: The contents of serum RF, CCP antibody, Ig (IgM and IgG, IgA) of RA group (activity and remission) were respectively significantly higher than that of the control group (P<0.05, or P<0.01). The contents of serum levels of IL-1β, TNF-α, IFN-γ and IL-4 of RA group (activity and remission) were respectively significantly higher than that of the control group (P<0.05, or P<0.01). The intra-group comparison between activity group and remission group in RA showed no significant difference in IL-4 level (P>0.05). The levels of cytokine IL-1β, TNF-α, IFN-γ were positively correlated with RF, CCP antibody, Ig and DAS28 scores in RA activity while which were only positively correlated with DAS28 scores of RA in remission, and the IL-4 level had no significant correlation with other indicators. Conclusions: The detection of levels of RF, CCP antibody, Ig and cytokine for the diagnosis, treatment and prognosis of RA has important reference value