Evaluation of Anti-Oxidant Activity of Lavandula angustifolia using DPPH Method

Abstract Background: Stress oxidative factors are known to causes diseases resulting from metabolic disorders. Therefore, preventing, or at least decreasing the amount of these factors may have a positive impact on prevention or improvement of the metabolic problems. Recently, the herbal medicines are more considered due to more effectiveness. We designed the present study to evaluate anti-oxidant effect of aqueous extract of lavender (Lavandula angustifolia). Materials and Methods: For extract preparation, the dry aerial part of lavender mixed with boiling water for 4 hours and then the container was filtered and condensed in a bain marie. Finally, the extract was powdered by freeze dryer. The anti-oxidant activities of the herbal medicine samples in 5, 10, 20 and 40 ppm concentrations were determined via DPPH method. This method is based on free radical scavenging of 2, 2- dipheny L-1-picrylhydrazyl(DPPH) reflected in the color and absorbance changes in spectrophotometery method in 520 nm. Finally the IC50 was calculated and compared with that of for vitamin C as a standard. Results: All doses of the aqueous extract of the lavender showed dose- dependent potent anti-oxidant activity, So that, their differences were significant compared to control sample. The IC50 of the herbal medicine was 24.66 ppm that was less than the vitamin C of 2.3 ppm. Conclusion: As a potent anti-oxidant, the lavender aqueous extract can be effective in treatment of metabolic diseases