Microbiological study of root canal content in dynamics of treatment of destructive forms of periodontitis

Aim. Assessment of the efficacy of complex endodontic treatment based on microbiological study of dental root canal content. Methods. 92 patients with destructive forms of chronic periodontitis aged 25-35 years, without endodontic intervention in the anamnesis, underwent endodontic treatment. Depending on the conducted treatment all patients were divided into two groups: in group 1 complex treatment was performed on 52 teeth based on our developed scheme, group 2 underwent treatment of 40 teeth with the use of standard treatment method. The microbiological study of root canal content was conducted before treatment and was repeated before sealing with permanent sealing material. Results. The microbiological study of root canal content before treatment was characterized by significant variety of microbial flora, high prevalence of bacterial and fungal flora, its content was 4-5.8 lg CFU/ml. The most aggressive facultative anaerobic Gram-negative and Gram-positive bacteria were observed: Prevotella intermedia, Fusobacterium, Actinomyces spp. The repeated microbiological study after complex endodontic and standard treatment of destructive forms of periodontitis demonstrated reduction of the quantity of bacteria in root canal. The developed treatment scheme turned out to be more effective compared to the standard method. In root canals only some species were found, and in isolated cases Streptococcus intermedius, Streptococcus sangius, Enterococcus spp. were isolated in extremely small quantities, not more than 3.0 lg CFU/ml (103 CFU/ml). Conclusion. Use of the complex endodontic and standard treatment of destructive forms of periodontitis resulted in the decrease of bacterial content and variety of species in root canal; higher microbiological efficacy was demonstrated for the suggested complex treatment method compared to the standard method