Myostatin negatively regulates NCM growth and proliferation by modulating signaling through Akt and NFAT3.

<p>NCM's were cultured as described above. Cells were treated with Ad-CMV-LacZ (control), Ad-CMV-dnMSTAT, Ad-CMV-MSTAT at MOI = 100 for 18 hours, then stressed with PE (100 uM) for 0 or 15 minutes. Each condition was tested n = 4 times. Phospho-specific Western blot was performed to assess the activation states of (A) Akt and (B) NFAT3. *p<0.05 vs. LacZ Con (ANOVA).