Protein expression levels of parasites treated with <i>Sm-tsp-2</i> dsRNA.

<p>Protein extracts from adult parasites (A) and schistosomula (B) treated with <i>Sm-tsp-2</i> or <i>luciferase</i> dsRNAs for 7 days were loaded onto a 12% SDS-PAGE gel at 2, 1, 0.5 and 0.25 µg. Proteins were transferred onto nitrocellulose and immunoblotted with anti-<i>Sm</i>-TSP2 or anti-<i>Sm</i>-Pmy monoclonal antibodies. The intensity of paramyosin expression was evaluated to determine equal protein loading.