Mutations in the <i>lasR</i> gene (A) and <i>rhlR</i> gene (B) of <i>P. aeruginosa</i> isolates from CF patients.

<p>The nucleotide sequence alterations were identified by alignment with the PAO1 sequence. On top of the PAO1 sequence the nucleotide substitutions, insertions (ins) or deletions (Δ) are indicated. Under the amino acid sequence, frame shifts (fs), stop codons(*) or amino acid deletions (Δ) or changes are indicated in bold. The altered nucleotides are shown in yellow if the mutation was complemented with a plasmid containing the wild-type <i>lasR</i> or in grey if not complemented. Amino acid changes that have been previously shown to impair the LasR function <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010115#pone.0010115-Heurlier1" target="_blank">[37]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010115#pone.0010115-Kiratisin1" target="_blank">[38]</a> are marked in squares and amino acid changes that have been previously described in <i>in vitro</i> studies <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010115#pone.0010115-DArgenio1" target="_blank">[22]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010115#pone.0010115-Bottomley1" target="_blank">[39]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010115#pone.0010115-Kovach1" target="_blank">[48]</a> are encircled.