Anti-IgY Abs do not block neutralizing activity of virus specific IgY.

<p>PR8 virus neutralizing activity of PR8 specific IgY (anti-PR8 IgY) in the absence of anti-IgY serum was determined by microneutralization assay. VN titer of anti-PR8 IgY is 1∶320 at which the viral nuclear protein (NP) was not detected (Fig. 5A). In the presence of anti-IgY serum VN by anti-PR8 IgY was not abrogated by incubation with normal serum (Fig. 5B and 5D) or with anti-IgY serum (Figs. 5C and 5D). VN titer (1∶320) of anti-PR8 IgY was used in the assay. The optical density (O.D.) was determined at 450 nm (<i>A</i>₄₅₀).