Effects of mutated GNE^M712T on ST3Gal5 mRNA expression.

<p><i>A</i>, Comparison of GNE mRNA levels between <i>Gne^+/+ (diamond)</i> and <i>Gne^M712T/M712T (square)</i> mouse muscle determined by qRT-PCR (absolute values are expressed as the number of GNE mRNA copies/µg of total RNA). <i>B</i>, ST3Gal5 mRNA levels <i>Gne^+/+</i> and <i>Gne^M712T/M712T</i> mouse muscle (absolute values are expressed as the number of GNE mRNA copies/µg of total RNA). (*, <i>p<0.025</i>); each data point represents a minimum of 3 replicates). Mean ST3Gal5 expression in <i>Gne^M712T/M712T</i> muscle was 64.41%±10% of <i>Gne^+/+</i> muscle, whereas no significant difference could be detected in GNE expression.