Kinetics of insulin-induced Akt phosphorylation and inhibitory effect of Ang II on insulin-induced Akt activation.

<p>L6 myotubes were exposed to insulin (100 nM) for 0–120 min. Blots were probed with anti-Akt and anti-phosphoSer⁴⁷³-Akt (<b><i>A</i></b>) or anti-phosphoThr³⁰⁸-Akt (<b><i>B</i></b>). L6 myotubes were pretreated with Ang II 10 nM for 30 min and stimulated with insulin (100 nM) for 60 min. Blots were probed with anti-Akt and anti-phosphoSer⁴⁷³-Akt (<b><i>C</i></b>) or anti-phosphoThr³⁰⁸-Akt (<b><i>D</i></b>) antibodies. Results are expressed as fold change of the ratio of phospho-Akt/Akt over controls (means ± s.d.) and the blots are representative of three independent experiments (*<i>P</i><0,05 vs control, °<i>P</i><0,05 vs Ang II, ^#<i>P</i><0,05 vs Ang II + insulin).