Deficiency of murine P2Y₁₂ receptors affects thrombus formation on plaque at high shear rate.

<p>Citrate-anticoagulated blood from P2Y₁₂^−/− and corresponding wild type (P2Y₁₂^+/+) mice was supplemented with OG488-fibrinogen (25 µg/ml) and co-perfused with ADP (10 µM) over homogenized murine plaque material at 1000 s⁻¹ for 4 min. (A) Representative phase-contrast and fluorescence images (bar = 20 µm). (B) Average size of thrombi after perfusion. (C) Average fluorescence intensity (arbitrary units) from fibrin(ogen)-binding platelets and thrombi. (D) Histograms of features on surface; estimated numbers of platelets per feature were 9–24 (white), 24–75 (light gray), 75–400 (dark gray) and >400 (black). (E) Disaggregation events measured per platelet aggregate per min. Data are means ± SE (<i>n</i> = 3), *<i>p</i><0.05.