Effect of DPT-AIF peptides on cell survival and cell death.

<p><u>Cell viability assay</u>: (A) HeLa cells, or (B) other adherent cell lines, were incubated for 24 hours with DPT-AIF peptides prior to processing for MTT staining. In A, SD is shown for n = 3, in B for simplicity the figure corresponds to a representative experiment repeated three times. <u>Cell death assay</u>: (C) HeLa cells were incubated for 2 hrs with DPT-AIF peptides prior to processing for the detection of TUNEL positive cells. The % of positive cells was obtained by ANOVA test and significance was set at P<0.05. (D) To monitor apoptosis in Jurkat cells treated with DPT-AIF peptides, we used Annexin V (left panel) or DiOC₆ (right panel) assays as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0009981#s2" target="_blank">Methods</a>. Statistical analysis was by ANOVA and significance was set at P<0.05.