Monocytes and T cells are targets for NK cell mediated lysis.

<p>Highly purified NK cells were left untreated or treated with anti-CD16 mAb (3 µg/ml), IL-2 (1000 u/ml), or a combination of IL-2 (1000 u/ml) and anti-CD16 mAb (3 µg/ml) for 8–12 hours before they were added to the ⁵¹Cr labeled DPSCs, or ⁵¹Cr labeled monocyts or ⁵¹Cr labeled T cells at different effector to target (E:T) ratios. After 4 hours of incubation the supernatants were removed and the released radioactivity counted by a γ counter. % cytotoxicity was determined at different E:T ratio, and LU₃₀/10⁶ cells was calculated using the inverse of the number of effectors needed to lyse 30% of either DPSCs, monocytes or T cells X100. One of three representative experiments is shown in this figure.