<p>Representative histogram plots on one NKT cell clone showing (A) surface expression of CD69 and (B) TCR detection by CD1d TM following 16 hours <i>in vitro</i> stimulation with the NKT-specific ligand α-GalCer presented on C1R cell lines expressing CD1d (C1R.d/α-GC), C1R cell lines pulsed with α-GalCer (C1R/α-GC), PMA/Ca or medium alone (R10).</p
<p>PBMC were isolated and co-cultivated for three hours with 293-mock or 293-LLT1 transfectants. Act...
<p>A) Representative contour plots showing surface expression of CD4 and CD8 molecules on <i>ex vivo...
<p>As shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0131477#pone.01...
<p>A) IFN-γ ELISA on culture supernatants of NKT clones stimulated with C1R.d/α-GalCer (50 ng/ml) fo...
<p>A) Intracellular cytokine staining of one representative NKT clone with antibodies against IFN-γ,...
<p>A) Histogram plots gated on representative NKT clones showing surface expression of NK cell marke...
<p>A) Representative dot plots showing gating strategy for identification of NKT lymphocytes. On the...
<p>A) Cytokine ELISA for IFN-γ, IL-2, IL-10, IL-13, and IL-4 with culture supernatants collected 24 ...
Activation of NKT cells using the glycolipid α-galactosylceramide (α-GalCer) has availed many invest...
<p>Purified DN (filled symbols) and CD4 (open symbols) T cells isolated from ten uninfected magabeys...
<p>(<b>A</b>) Quantitative real-time PCR analysis of DGK-α, ζ and δ mRNA in sorted CD8 cαβT cells an...
<p>(A) Gating strategy (upper panel) for NK cells (TCRβ<sup>−</sup>NK1.1<sup>+</sup>DX5<sup>+</sup>)...
<p><b>(A)</b> Representative plots of NKT cell localization, activation, and IFN-γ production after ...
<div><p>Natural killer T (NKT) cell development depends on recognition of self-glycolipids via their...
<p>(A, B) To test the lysis of ligand-loaded target cells, the cytotoxic activity of γδT (A) or γδT-...
<p>PBMC were isolated and co-cultivated for three hours with 293-mock or 293-LLT1 transfectants. Act...
<p>A) Representative contour plots showing surface expression of CD4 and CD8 molecules on <i>ex vivo...
<p>As shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0131477#pone.01...
<p>A) IFN-γ ELISA on culture supernatants of NKT clones stimulated with C1R.d/α-GalCer (50 ng/ml) fo...
<p>A) Intracellular cytokine staining of one representative NKT clone with antibodies against IFN-γ,...
<p>A) Histogram plots gated on representative NKT clones showing surface expression of NK cell marke...
<p>A) Representative dot plots showing gating strategy for identification of NKT lymphocytes. On the...
<p>A) Cytokine ELISA for IFN-γ, IL-2, IL-10, IL-13, and IL-4 with culture supernatants collected 24 ...
Activation of NKT cells using the glycolipid α-galactosylceramide (α-GalCer) has availed many invest...
<p>Purified DN (filled symbols) and CD4 (open symbols) T cells isolated from ten uninfected magabeys...
<p>(<b>A</b>) Quantitative real-time PCR analysis of DGK-α, ζ and δ mRNA in sorted CD8 cαβT cells an...
<p>(A) Gating strategy (upper panel) for NK cells (TCRβ<sup>−</sup>NK1.1<sup>+</sup>DX5<sup>+</sup>)...
<p><b>(A)</b> Representative plots of NKT cell localization, activation, and IFN-γ production after ...
<div><p>Natural killer T (NKT) cell development depends on recognition of self-glycolipids via their...
<p>(A, B) To test the lysis of ligand-loaded target cells, the cytotoxic activity of γδT (A) or γδT-...
<p>PBMC were isolated and co-cultivated for three hours with 293-mock or 293-LLT1 transfectants. Act...
<p>A) Representative contour plots showing surface expression of CD4 and CD8 molecules on <i>ex vivo...
<p>As shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0131477#pone.01...