<p>A) Cytokine ELISA for IFN-γ, IL-2, IL-10, IL-13, and IL-4 with culture supernatants collected 24 hours post-stimulation of PBMC (mean and SEM of 4 to 7 animals). B) Kinetics of cytokine secretion in PMA/Ca and C1R.d/α-GC stimulated PBMCs at 2 hour, 1 day, 2 days, and 7 days post-stimulation. C) IFN-γ production in C1R.d/α-GC stimulated lymphocytes in the presence of anti-human CD1d (clone 42.1) blocking antibody or isotype control Ab.</p
<p>Peripheral blood mononuclear cells were stimulated for 18 hours and supernatants were assayed for...
<p>(a–d) The amount of IFNγ, IL-4, IL-10, and IL-17 secreted into the supernatant was measured by EL...
<p>Data are shown as spot forming cells per million peripheral blood mononuclear cells (SFC /10<sup>...
<p>Cytokine ELISA for IFN-γ, IL-2, IL-13, IL-10, IL-6, and TGF-β with culture supernatants collected...
<p>A) Intracellular cytokine staining of one representative NKT clone with antibodies against IFN-γ,...
<p>A) IFN-γ ELISA on culture supernatants of NKT clones stimulated with C1R.d/α-GalCer (50 ng/ml) fo...
<p>A) Representative flow cytometry plot of IFNγ protein expression (x-axis) and IL4 protein express...
<p>A) Representative dot plots showing gating strategy for identification of NKT lymphocytes. On the...
<p>Purified DN (filled symbols) and CD4 (open symbols) T cells isolated from ten uninfected magabeys...
<p>Freshly isolated peripheral blood mononuclear cells (PBMC) from ten donors were left unstimulated...
<p>Expression of IL2 receptor (CD25) and IL7 receptor (CD127) was assessed in memory population (CD9...
<p>(A) PBMCs from SIV-negative SMs and SIV-negative RMs were incubated for 18 hr with 10 µM CL097 or...
Lack of chronic immune activation in the presence of persistent viremia is a key feature that distin...
<p>Peripheral blood cultures were collected after 7 days in culture with recombinant canine IL-4 and...
<p>PBMC were isolated one month after ovalbumin booster injection, stimulated in vitro with ovalbumi...
<p>Peripheral blood mononuclear cells were stimulated for 18 hours and supernatants were assayed for...
<p>(a–d) The amount of IFNγ, IL-4, IL-10, and IL-17 secreted into the supernatant was measured by EL...
<p>Data are shown as spot forming cells per million peripheral blood mononuclear cells (SFC /10<sup>...
<p>Cytokine ELISA for IFN-γ, IL-2, IL-13, IL-10, IL-6, and TGF-β with culture supernatants collected...
<p>A) Intracellular cytokine staining of one representative NKT clone with antibodies against IFN-γ,...
<p>A) IFN-γ ELISA on culture supernatants of NKT clones stimulated with C1R.d/α-GalCer (50 ng/ml) fo...
<p>A) Representative flow cytometry plot of IFNγ protein expression (x-axis) and IL4 protein express...
<p>A) Representative dot plots showing gating strategy for identification of NKT lymphocytes. On the...
<p>Purified DN (filled symbols) and CD4 (open symbols) T cells isolated from ten uninfected magabeys...
<p>Freshly isolated peripheral blood mononuclear cells (PBMC) from ten donors were left unstimulated...
<p>Expression of IL2 receptor (CD25) and IL7 receptor (CD127) was assessed in memory population (CD9...
<p>(A) PBMCs from SIV-negative SMs and SIV-negative RMs were incubated for 18 hr with 10 µM CL097 or...
Lack of chronic immune activation in the presence of persistent viremia is a key feature that distin...
<p>Peripheral blood cultures were collected after 7 days in culture with recombinant canine IL-4 and...
<p>PBMC were isolated one month after ovalbumin booster injection, stimulated in vitro with ovalbumi...
<p>Peripheral blood mononuclear cells were stimulated for 18 hours and supernatants were assayed for...
<p>(a–d) The amount of IFNγ, IL-4, IL-10, and IL-17 secreted into the supernatant was measured by EL...
<p>Data are shown as spot forming cells per million peripheral blood mononuclear cells (SFC /10<sup>...