Fluorescence detection of SEB binding on an array with Ppy deposited using constant voltage.

<p>Polypyrrole was deposited using potentials from 0.0 to 2 V for 0.5, 1.0, 2.0, or 5.0 s followed by adsorption of anti-SEB MAb. Different concentrations (0.0, 0.1, 1.0 or 10.0 pg/ml) of SEB were incubated in individual chambers of a four-chamber hyb cap, and binding was detected using biotinylated rabbit anti-SEB with Cy5-SA.