FcRγ expression in monocytes of HIV-infected and uninfected individuals.

<p>FcRγ mRNA (A) and protein (B) were determined by Q-PCR and quantitative infrared immunoblotting in extracts from monocytes purified by FACS from blood of HIV-1 infected (individuals 1–18, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0009643#pone-0009643-t001" target="_blank">Table 1</a>) and control subjects. Fluorescence from FcRγ immunoblots measured at 680 nm was normalised to fluorescence of GAPDH in the same immunoblots, measured at 800 nm. FcRγ mRNA was determined from real-time PCR measurements using the comparative threshold method with GAPDH mRNA serving as internal control. Differences between groups were tested using the Mann-Whitney U test for non-parametric data, with a value &lt;0.05 assumed to be significant. Horizontal bars represent median values.