Structural organization of <i>sigV, oatA, dltA</i> and <i>mprF</i> loci.

<p>Large arrows correspond to the indicated genes. Grey areas show the deleted region by double cross over event and that of <i>sigV</i> locus (harbouring an asterisk) represents the deletion carried out in SAS mutant strain. The solid triangle positioned on EF0031 shows the insertion site of the integrative pUCB300-<i>mprF</i> recombinant plasmid generating the <i>mprF</i> mutant. The putative promoters and terminators are indicated with P and T letters, respectively. Primers used to construct the different mutants are indicated by black arrows, and listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0009658#pone-0009658-t004" target="_blank">Table 4</a>.