Mating effects on cuticular compounds.

<p>(A & C) Global effects. Data shown represent the mean (± SEM) for the relative abundance of cuticular compounds in 4-day-old virgin (empty bars) and in 6-day-old mated (filled bars) males (A) and females (C). <i>n</i> = 6. (B & D) Individual effects. Data shown represent the mean (± SEM) for the <i>Post/Ante</i> ratio (after/before mating) calculated for each compound in individual males (B) and females (D). The confidence limits of the <i>Post∶Ante</i> ratios calculated for the mating effect (shaded stripe) ranges from 0.968 to 1.212 in males, and from 1.037 to 1.253 in females. The compounds in parentheses were not detected in either sex; those shown within a frame appeared during mating. The numbers inside the circle (above the baseline) indicate the proportion of individuals in which they appeared; the grey circles labelled with “3/6” indicate the compounds that appeared in only 50% of mating females. For statistics, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0009607#pone-0009607-g002" target="_blank">fig. 2</a>.