Thermal stability of metallated and demetallated wildtype SOD1, SOD1^G93A and GFP-SOD1^G93A proteins.

<p>The thermal stability of wildtype SOD1 and SOD1^G93A mutants was assessed at two concentrations (11µM and 33µM) and in the metallated and demetallated form (when incubated with 20mM EDTA which functioned as a heavy metal chelator). Protein stability is expressed as apparent Tm, which refers to the mid-point temperature (°C) where the ratio of unfolded to folded proteins is 1∶1. Apparent Tm values reported are the average values±SD obtained from n = 3. Asterisks denote p-values calculated using the two-tailed Student t-test for the paired condition of metallated versus demetallated SOD1, where ** = p<0.005 and *** = p<10⁻⁵.