Placental <i>Angpt2</i> mRNA expression is increased by malaria infection (<i>P</i> = 0.0063, 2-way ANOVA).

<p><i>(A</i>) <i>Angpt1</i> and <i>(B</i>) <i>Angpt2</i> transcripts were measured by real-time quantitative PCR using cDNA templates reverse transcribed from placental RNA from pregnant mice uninfected (light bars) and infected with <i>P. berghei</i> (dark bars). Copy number was normalized to housekeeping gene expression as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0009481#s2" target="_blank">Materials and Methods</a>. <i>(C)</i> The relative expression of <i>Angpt2</i>/<i>Angpt1</i> is also increased in placentas associated with viable fetuses of infected mice (<i>P</i> = 0.0032, 2-way ANOVA on log-transformed data). Dots are individual placentas associated with viable fetuses; bars represent the median of each group. 4–6 mice are represented per group. *<i>P</i><0.01 (Bonferonni post-test). D, day post infection/control injection; G, gestational day.