Selectable lentiviruses with Wnt-induced eGFP.

<p>A) Schematic description of the 7xTcf-eGFP//SV40-mCherry (7TGC) and 7xTcf-eGFP//SV40-Puro^R (7TGP) lentiviruses. LTR: Long Terminal Repeat, ¬: packaging signal, RRE: Rev Response Element, cPPT: central PolyPurine Tract, WPRE: Woodchuck hepatitis Post-transcriptional Regulatory Element, dPPT: distal PolyPurine Tract, SIN: Self Inactivated (LTR). B) HEK293 or mouse L cells were infected with the 7TGC lentivirus. All infected cells expressed mCherry and addition of purified Wnt3A protein (500 ng/ml final, “Wnt3A”) led to expression of eGFP. In absence of Wnt signal (“vhc”), eGFP was not expressed. C) HEK293T cells were infected with the 7TGP lentivirus and either selected in 1 µg/ml puromycin (“7TGP+puro”) or grown in absence of drug (“7TGP”). Expression of eGFP was induced by Wnt3A-conditioned medium (“Wnt3A”) and Wnt-responsive cells were strongly enriched after puromycin.