Lack of FoxM1 expression increased apoptosis of DP thymocytes in culture.

<p>Left panel: apoptosis of DP thymocytes (Annexin V⁺ 7ADD⁻) culture <i>in vitro</i> for 24 hr. Middle panel: total number of cell death as measured by Annexin V⁺ 7ADD⁺ of DP thymocytes culture <i>in vitro</i> for 24 hr. Right panel: DP thymocytes from Lck-FoxM1 mice or their wild-type littermate controls were stimulated with anti-CD3/CD28, anti-Fas antibody, PMA phorbol ester (PMA) or dexamethasone (Dex). The extent of apoptosis (% Annexin V positive, 7-AAD negative cells) was measured 24 hours later.