Differential localization of V-ATPase B1/2 subunits following stimulation of cells with lung surfactant secretagogues.

<p>(A) Overnight cultured cells were stimulated with a combination of secretagogues 200 µM ATP and 0.2 µM PMA for 2 hrs. Immunostaining was performed to detect the localization of V-ATPase B1/2 subunit. The cells were also stained for the LB-180 protein to check for any changes in the lamellar body membrane following stimulation. Shown are the representative images. Scale bar: 40 µm. (B) Equal amounts of the total proteins (25 µg) isolated from control (Con) and stimulated (Sti) cells as in Panel A were immunobloted for V-ATPase B1/2 subunit and reprobed with β-actin. Shown are the representative immunoblots from 4 independent cell preparations.