Overview of the BAP mapping method.

<p>(a) A genomic BAC library is pooled in a 3-dimensional fashion (1^st D for plate ID; pool all unique clones for each plate and create overlapping superpools of plate pools; 2^nd D for row ID: pool all clones in identical rows over several plates [3.5 plates for <i>Arabidopsis</i>] and create overlapping ‘superpools’ of row pools to allow row identification; 3^rd D pool for column ID: pool all clones in identical columns over several plates [3.5 plates for <i>Arabidopsis</i>] and create overlapping ‘superpools’ of column pools to allow column identification) to generate the BAC-range panel. (b) Of 96 wells of a plate, 88 contain random aliquots of a BAC library (8 wells are reserved for controls), each BAC aliquot contains DNA corresponding 0.6–0.7 fold coverage of the genome. The presence of single copy STS markers (M1-My) is indicated by various colours in the aliquots. (c) The presence of markers found after MT-PCR-HRM. (d) The BAC range panel mapping results allow creation of short linkage maps, and (e) at the same time, to establish a corresponding BAC tiling path. (f) To link the shorter contigs obtained from the BAC panel and to close the gaps between contigs, markers chosen from the ends of the contigs are mapped by the long-range (large size) DNA panel. (g) The merged linkage and physical map as the final result.