Localization of the A_2BR.

<p>Lung sections were stained with antibodies against the A_2BR. (A) Lung section from a Stage 0 COPD patient. (B) Lung section from a Mild IPF patient. (C) Lung section from a Stage 4 COPD patient. (D) Lung section from a Severe IPF patient. Sections are representative of 10–14 different patients from each group. Scale bars = 100 µm. (E) A_2BR positive inflammatory cells were quantified in 20 images. Data are presented as mean number of positive cells per 10X field ± SEM. *p≤0.05 versus Stage 0 COPD. #p≤0.05 versus Mild IPF. n = 4 (Stage 0 COPD), n = 10 (Mild IPF), n = 8 (Stage 4 COPD and Severe IPF). (F) A_2BR expression in hyperplastic airway epithelial cells (blue arrow) and fibroblasts (red asterix). Scale bar = 200 µm.