SM induces the last round of premeiotic DNA synthesis in Kit positive spermatogonia.

<p>A) Kit positive spermatogonia from 7 dpp mice were cultured for 24 h under gravity and SM conditions in the presence of BrdU and analysed by flow cytometry for BrdU incorporation and propidium iodide labeling. The upper quadrants show the limits for replicating cells. Percentages of cells in the different states are indicated. BrdU, bromodeoxyuridine. B) Representative immunofluorescence images of Scp3-positive (red) and BrdU-stained (green) premeiotic S-phase nuclei (preleptotene). Scale bar, 10 µm. C) Histogram showing the percentage of double positive cells respect to total BrdU stained nuclei after 24 of culture under gravity or SM condition. The values are means ± SD of three separate experiments. P≤0.05.