Electron microscopy images of pSer9GSK3β labeling in cortical and subcortical areas.

<p><b>A)</b> pSer9GSK3β labeling in a neuron in the piriform cortex. The labeling is located in the rough endoplasmic reticulum (outlined arrows), free ribosomes (stars) and the outer mitochondrial membrane (black arrow). Note the strong staining in a cluster of free ribosomes in <b>A inset</b>. <b>B)</b> pSer9GSK3β immunolabeling in a microglia cell in the piriform cortex. Staining is present in the rough endoplasmic reticulum (outlined arrows) as well as in free ribosomes (stars). <b>B inset</b> shows a detail of the labeling present in this cell. <b>C)</b> pSer9GSK3β labeling in another microglia cell in the piriform cortex. In this case labeling is prominently present in the nucleus (arrowheads) while the cytoplasmic labeling is restricted to a small portion of the rough endoplasmic reticulum (outlined arrow). <b>C inset</b> shows a detail of the labeling present in the nucleus (arrowhead) and the rough endoplasmic reticulum (outlined arrow) in the proximity of the outer nuclear membrane. <b>D)</b> pSer9GSK3β staining in a microglia cell in the striatum. Labeling is prominent in clusters of free ribosomes (stars) in the cytoplasm as well as in the process (see <b>D inset</b>). <b>E)</b> Photomicrograph of pSer9GSK3β staining in a neuron in the motor cortex. Note the prominent labeling in the nucleus (arrowheads). Labeling is also present in the rough endoplasmic reticulum (outline arrows, see also <b>E Inset</b>), as well as in clusters of free ribosomes and in the outer membrane of one mitochondria (black arrow). Note also the presence of an astrocytic process (a) showing immunolabeling in free ribosomes (star). <b>F)</b> Detail of an astrocytic process (a) showing pSer9GSK3β immunolabeling in rough endoplasmic reticulum (outlined arrow), free ribosomes (star) and mitochondria (black arrows). [Lf: Lipofuscin body; N: nucleus; <u>Scale bars</u>: 1 µm in A–C, E; 0.5 µm in D; 0.25 µm in B inset, C inset; 0.1 µm in D inset]