Effect of HA on breast cancer cell adhesion.

<p>(A) Analysis of von Kossa stained histological cross-sections indicates that MDA-MB231 cells (stained red) penetrate into the center of mineralized scaffolds (HA stained black), while this is not the case for non-mineralized control scaffolds. Arrows and asterisks indicate representative scaffold walls and pores, respectively. Scale bars represent 200 µm. (B) MDA-MB231 breast cancer cells adhere more efficiently to mineralized scaffolds than non-mineralized scaffolds (**p<0.01), and pre-incubation of MDA-MB231 cells with RGD peptide inhibits enhanced adhesion to mineralized scaffolds (**p<0.01). Error bars are small where not visible. (C) Fibronectin adsorption within the polymer scaffold is increased in mineralized scaffolds (lane 2) relative to non-mineralized scaffolds (lane 1) as indicated by Western Blot analysis of scaffold lysates.